The Effect of the Catalase on Hydrogen Peroxide

The Effect of the Catalase on Hydrogen Peroxide
Plan What is my Investigation about? In my investigation I will be investigating the effect of the Catalase on Hydrogen peroxide, I no that the higher the volume of the Catalase the faster it will react. For this reason I will be using a volume of 20 vol, from our preliminary experiment we found out that using this volume will give us the best set of results and will be easier to record the amount of gas given off. Catalase [image]Hydrogen Peroxide Water + Oxygen [image] H2O2 H2O + O2 What Can I Change? In my experiment there are a number of things I could change, e.g, the amount of H2O2 I use, I could use 10ml, 20ml, 30ml, 40ml and 50ml, in our preliminary experiment we found out that the more H2O2 we use the slower the reaction will happen this will help us in our experiment as we will need a medium rate of gas given off to insure our results are accurate.
We Could also change the surface area of the liver to make the reaction happen at different speeds as we found out that if the exposed surface is larger then there are more enzymes exposed to the hydrogen peroxide, because of this there are more enzymes reacting at the same time which will make the test work faster, we found that if you have a liver solution the surface area is a lot bigger then a solid piece of liver and it is also a lot easier to mix the solutions together to get them to react better. I could change the amount of liver I use, I could use 20g 25g 30g or even 35g, I predict that the more of liver there is, the quicker it?ll work, this is because the more liver solution/ liver there is the faster the enzymes will react as there is more enzymes for the hydrogen peroxide to react with. I can change the concentration of the H2O2. I predict, the lower the volume, the longer it?ll take longer as there is less enzyme proportional to the hydrogen peroxide which will cause it to work slower. I could change the temperature I perform the experiment at. I could use the following temperatures 30? 33? 36? 39? 42? and 45?C. This will effect the time for the catalase to work as we found out in our preliminary experiment that the enzymes work the best at 37? this is because our bodies are at 37? and the enzymes work best at that temperature so testing temperatures bellow and above 37? will show how the enzymes react to different temperatures and will give us a clear set of results. Final Choice/ what I will change. I decided to change the temperature because it will give me the best set of results and will be easier and fairer then the rest of the options. As we already know that enzymes work best at 37?, we would also like to see how they react to lower and higher temperatures so we will have the following temperatures 31? 34? 37? 40? 43? and 46?C, we also know that the enzymes denature at around 45?C so we would also like to test if this theory is true by testing it at 46?C. For a table and a graph refer to attachment. Amount of Liver Solution Amount of Bubbles 5ml 150 4ml 150 3ml 150 2ml 150 1ml 100 0.5ml 45 What will I keep the same for fairness? In my experiment I will keep the following the same: ? The pH, we will keep this at PH2 as the enzymes in the liver solution will work best at that ph. ? Concentration of H2O2, we will keep this the same as changing this will make the experiment unfair and changing the concentration will also make our results change, to what we expected. ? Amount of catalase, we will keep this the same because if we were to change it then it would effect the rate of reaction drastically, and will cause our predictions to be way off what we expect. ? Amount of H2O2, we will keep this the same because if we added more H202 then it would make the reaction slower and if we added less it would speed the reaction up and this would make it very hard for us to record the results. ? We will keep the surface area of the liver the same as we will make a solution with water and liver, we made this solution by adding liver to 1000ml of water and blending it, this made it a lot easier to keep it fair and it is also easier to measure the right amount of solution. Keeping these the same will keep my experiment fair as they will insure that only one thing will change to make sure it is fair. What Will I Measure And How? In my experiment I will measure the amount of gas given off, to measure this I will use a burette filled with water to catch the air bubbles, the bubbles will then push out the water leaving the carbon dioxide in the burette, although we will be using this method there are other methods which can be used like counting the bubbles but doing this is harder as you will have to count every bubble and not lose count, you will also have to take into account that not every bubble will be the same size, one may be bigger then another one, for these reasons we will use the burette as it is very accurate at measuring small amounts and will be a lot more accurate. For a diagram refer to attachment. In my experiment I will also need to take the temperature of the liver solution, to do this I will need a thermometer and I will record the results like I previously did in my preliminary experiment. What I Think Will Happen And Why? In our experiment there are many possible results but we are expecting the following to happen ? We will be expecting the lower temperature enzymes to work very slowly (slower then normal) ? We will be expecting the enzymes at around 37? to be working the fastest and best as this temperature is the temperature that the enzymes work best at. ? We will be expecting the enzymes at temperatures above 39?C to work slow but we will also expect the enzymes around 45?C to not be working at all this is because at 45?C and over the enzymes do no longer work they are denatured. How will I make sure that my experiment is safe? To make sure our experiment is safe we will do a number of things to insure no one is hurt, the following what we will do to insure safety. ? We will wear goggles to make sure none of the hydrogen peroxide gets in our eyes as it will damage them. ? We will wash our hands after the experiment as if we had hydrogen peroxide on our hands it could make its way into your mouth or eyes and cause damage. ? We will not sit on any chairs/stools during the experiment as if hot liquid was spilled it would likely land on our laps. ? We will use tongues to pick up hot test tubes.

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