Effect Of Detergent Concentration On Membrane Permeability Of Beetroot Cells

Effect Of Detergent Concentration On Membrane Permeability Of Beetroot Cells
Aim: To investigate how effect of Detergent Concentration (cont.) has on Membrane permeability of Beetroot cells. Hypothesis: I predict that as detergent concentration increases, the solution will become less clear, plus mass increases. The increases in mass will indicate that the water potential of the Beetroot cell is lower than that of the surrounding sucrose solution. The Beetroot discs will become flaccid and decrease in mass if the water potential of the surrounding solution is lower than the water potential inside the beetroot cell.



Cell Membranes Investigation

Cell Membranes Investigation
Aim To investigate the influence of a variety of treatments on the permeability of the cell membrane. This experiment aims to discover the effect of 6 different treatments on the permeability of the cell membrane. These will be distilled water, sucrose, sodium hydroxide, hydrochloric acid, ethanol and boiled water. The treatments include immersion in these solutions, and exposure to high temperature. The tissue used is from the taproot of a beetroot, and the effect on the membrane?s permeability assessed by the amount of pigment leakage that occurs. Hypothesis == I predict that the most damage to the membrane will be caused by the acid or the boiling water. I think that this will happen because generally hydrochloric acid causes more damage to things and also it produces more aggressive reactions causing greater effects this I think means that the acid is more likely to effect or disable the proteins in the lipid bi-layer this means that if this happens the membranes selective permeability will be lessened and the restriction of the flow of pigment out of the beetroot will also be lessened so more pigment can and will flow out.



Investigating the Effect of Different Solutions on Plant Cells

Investigating the Effect of Different Solutions on Plant Cells
The aim of my experiment is to find the effects of different solutions
on plant cells.

Osmosis is a form of diffusion, the movement of molecules to allow
them to mix, which occurs in plants. When a cell containing a weak
solution is next to a cell containing a stronger solution the water in
the weaker solution moves towards the stronger solution. This is why I
think that the pieces of potato in the concentrated solution will
decrease in both weight and length. However, I believe that the potato
will do the opposite and increase in length and weight.



Investigating the Effect of Different Solutions on Plant Cells

Investigating the Effect of Different Solutions on Plant Cells
The aim of my experiment is to find the effects of different solutions on plant cells. Osmosis is a form of diffusion, the movement of molecules to allow them to mix, which occurs in plants. When a cell containing a weak solution is next to a cell containing a stronger solution the water in the weaker solution moves towards the stronger solution. This is why I think that the pieces of potato in the concentrated solution will decrease in both weight and length. However, I believe that the potato will do the opposite and increase in length and weight. I cut 15 identical pieces of potato, measured and weighed them, and placed 3 in 0.25mol/l 3 in 0.50mol/l 3in 0.75mol/l 3 in 1.00mol/l and 3 in water inside a test tube. I ensured that each test tube had 10ml of liquid surrounding the potato. I gave it 3 days before weighing and measuring the potato again to see the difference.



The Effect of Sucrose Concentration on the Mass of Potato Tissue

The Effect of Sucrose Concentration on the Mass of Potato Tissue
Prediction: I predict that a high sugar solution will cause the mass of the potato to decrease. This will be due to osmosis. Osmosis is the diffusion of water from a region of high concentration to a region of low concentration across a semi-permeable membrane, such as the one of the potato. If the water concentration inside the potato is higher than the water concentration in the solution then the cell will lose water through osmosis, which will cause it to shrivel. When the plant cell is in said state it is plasmalised. However, if the plant cell is in a pure water solution, the plant will take in a large amount of water and has gains enough water, when a plant is in this state- it is said to be turgid. When this happens, the plant is said to be turgid. So, I predict that when the potato is in a strong sugar solution it?s mass will decrease. Equipment; Five test tubes ? To hold the potato and each one of the five sugar solutions individually; Test tube rack; Knife and equipment to cut potato into 5 pieces of equal mass; Measuring cylinder for measuring amount of water and finding the correct amount; Scales for weighing sugar; Thermometer to ensure the temperature of each solution is identical to the others; Spatula for stirring sugar solutions; Sugar and distilled water. Diagram [image] Method: Using a scalpel, cut five potato pieces. They should be of an almost equal size and mass.



Matthias Scheilden Essay

Matthias Scheilden EssayMatthias Scheilden was born on April 5, 1804. He was born in Hamburg, Germany. He died on June 23, 1881. He made a significant impact in science. He came up with a cell theory, which had a large effect of scientific attention to living processes as they happened on the cellular level. His work initiated the field of embryology.



The Effect of Different Temperatures on the Permeability of Beetroot Cell Membrane

The Effect of Different Temperatures on the Permeability of Beetroot Cell Membrane
Aim- to investigate the effect of different temperatures on the permeability of beetroot cell membrane. Prediction: I think that the higher the temperature the higher the absorbance of the liquid into the beetroot membrane. Table of results ==== Average temp (ºC) Group 1 absorbance (Arbitrary units) Group 2 absorbance (Arbitrary units) Group 3 absorbance (Arbitrary units) Average absorbance (Arbitrary units) 25 0.05 0.26 0.13 0.14 35 0.16 0.34 0.09 0.19 45 0.27 0.21 0.19 0.22 55 0.57 0.63 0.29 0.49 65 0.73 2 0.38 1.03 75 1.37 2 0.49 1.28 85 1.66 2 1.94 1.86 Statistical analysis ==== Spearman rank correlation -??????????? The reason for the statistical analysis is to find out if group 1, 2 and 3 and average absorbance is a positive or negative correlation or no correlation at all. Group 1 In group 1 each absorbance was increased when the temperature was increased so the rank was not changed therefore the rs was 1. This means it is over the critical value (0.79) therefore there is a positive significant correlation between the two values. Group 2 Site Temp (ºC) Rank R1 Absorbance (Arbitrary units) Rank R2 D (R1-R2) D2 1 25 1 0.26 2 -1 1 2 35 2 0.34 3 -1 1 3 45 3 0.21 1 2 4 4 55 4 0.63 4 0 0 5 65 5 2 6 -1 1 6 75 6 2 6 0 0 7 85 7 2 6 1 1 0 8 From using the formula the rs was 0.85, which is over the critical value (0.79) therefore there is a positive significant correlation between the two values. Group 3 Site Temp (ºC) Rank R1 Absorbance (Arbitrary units) Rank R2 D (R1-R2) D2 1 25 1 0.13 2 -1 1 2 35 2 0.09 1 1 1 3 45 3 0.19 3 0 0 4 55 4 0.29 4 0 0 5 65 5 0.38 5 0 0 6 75 6 0.49 6 0 0 7 85 7 1.94 7 0 0 0 2 From using the formula the rs was 0.96, which is over the critical value (0.79) therefore there is a positive significant correlation between the two values. Average absorbance In the average absorbance each absorbance was increased when the temperature was increased so the rank was not changed therefore the rs was 1. This means it is over the critical value (0.79) therefore there is a positive significant correlation between the two values



The Effect of Temperature on the Permeability of Beetroot Membrane

The Effect of Temperature on the Permeability of Beetroot Membrane
Analysis The graph shows the colorimeter readings increase as the temperature increases, they increase by the most at higher temperatures. This is shown by a smooth curve. This means that the beetroot samples release more dye at higher temperatures. This is because higher temperatures cause the membrane structure to break down. The membrane structure: Membranes have two layers of molecules called phospolipids to make up their structure. Phospholipis consist of a glycerol molecule plus two molecules of fatty and a phosphate group, this looks like a head with two legs, their head is attracted to water, this means theyÂ?re hydrophilic. The rest of the lipid (the fatty acid legs) is hydrophobic, which means they repel water. In aqueous solutions the phospolipids automatically arrange themselves into a double layer so that the hydrophobic legs are packed inside the membrane (away from the water) and the hydrophilic heads face outwards into the aqueous solutions. The molecules are represented in the Â?fluid mosaic modelÂ? shown below (the red bits are the legs of the phospolipids): [draw diagram here] If it was only made from phospolipids the membrane would be a barrier to water, this is why there are other components scattered throughout the phosphlipid by-layer. Glycolipids are lipids which have combined with polysaccharides.



Osmosis

Osmosis
These results show that a potato chip placed in water will gain in length, a weak sugar solution will lose length and a strong sugar solution will lose length also. The results from this test will allow me to choose an appropriate range of moralities in order to find out what the concentration is inside the cell vacuole. I am going to investigate 0.0, 0.2, 0.4, 0.6, 0.8 and 1.0 molar sugar solutions. I have chosen these concentrations to try and accurately find when there is no net movement of water, hence the concentration of the cell vacuole.



Investigating Osmotic Effects on Plant Cells

Investigating Osmotic Effects on Plant Cells
Introduction ==== Water is not only the medium in which metabolic reactions occur but it provides skeletal support and thus is vital for plants. Animal cells are not used to demonstrate osmotic effect in this study because it would raise moral and ethical issues in regards to animal rights and the use of animals in scientific experimentation. Also animal cells don?t have cell walls so if they were in dilute solutions the cell membrane would expand and eventually split. This type of change due to osmosis would be difficult to measure. A plant cell (fig 1) maintains its shape due to the cell wall being rigid and the cell wall pushing outwards onto it. Water will enter a cell by osmosis. Osmosis is the movement of water molecules from a dilute solution to a concentrated solution through a selectively permeable membrane (fig 2). When a plant cell gains water the vacuole expands causing the cell to stretch and become larger. The cell is turgid (fig 3). This occurs when the cell is surrounded by a more dilute solution. If a plant cell is put in a more concentrated solution then what is in, its vacuole becomes flaccid. The cell is plasmalysed. This is because water travels from a dilute solution to a concentrated solution by osmosis.